Journal: Cell metabolism

Article Title: Ejection of damaged mitochondria and their removal by macrophages ensure efficient thermogenesis in brown adipose tissue

doi: 10.1016/j.cmet.2022.02.016

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: CD86 Antibody, anti-mouse, FITC, Miltenyi Biotec , Miltenyi Biotec , Cat# 130–123-672; RRID:AB_2889633.

Techniques: Immunofluorescence, Recombinant, Cytometry, Purification, shRNA, Cell Culture, Lysis, XF Assay, Staining, Isolation, Transfection, Live Cell Imaging, Mouse Assay, Software, Expressing

Journal: Asian Pacific Journal of Cancer Prevention : APJCP

Article Title: A Comparative Study of the Bone Marrow- and Umbilical Cord-Derived Mesenchymal Stem Cells (MSCs) Efficiency on Generating MSC-Educated Macrophages (MEMs)

doi: 10.31557/APJCP.2022.23.9.3083

Figure Lengend Snippet: Antibodies Used to Analyze Surface Markers of MQs

Article Snippet: CD86 , FITC , Miltenyi Biotech, Germany , M1 MQ.

Techniques:

Journal: Asian Pacific Journal of Cancer Prevention : APJCP

Article Title: A Comparative Study of the Bone Marrow- and Umbilical Cord-Derived Mesenchymal Stem Cells (MSCs) Efficiency on Generating MSC-Educated Macrophages (MEMs)

doi: 10.31557/APJCP.2022.23.9.3083

Figure Lengend Snippet: Comparison of MQ CD Markers on Day 5, 7 and 10. Monocytes on day +5 express high level of CD11b/CD68 while expressing low level of CD80, CD86, CD206 and CD163. High expression of CD11b/CD68 indicated the monocyte differentiation to MQs during in vitro culture. Low expression of polarization markers also indicated a lack of differentiation into inflammatory/anti-inflammatory phenotype and, in fact, remaining the state of resting (M0) MQs. (A) Unstained cells; (B) Cells expressing CD11b/CD68; (C) Cells expressing CD80/CD86; (D) Cells expressing CD206/CD163. PE, phycoerythrin; FITC, fluorescein isothiocyanate

Article Snippet: CD86 , FITC , Miltenyi Biotech, Germany , M1 MQ.

Techniques: Comparison, Expressing, In Vitro

Journal: Oxidative Medicine and Cellular Longevity

Article Title: Anticancer Activity of Liquid Treated with Microwave Plasma-Generated Gas through Macrophage Activation

doi: 10.1155/2020/2946820

Figure Lengend Snippet: Depolarization of IL-4-pretreated Raw 264.7 macrophages at 24 h elapsed from PGNO-media treatment in various dilution ratios: (a, b) transcriptional level changes of macrophage M1 polarization-related genes, (a) including iNOS, IL6, and TNF- α , and (b) M2 polarization-related genes, including ARG, IL10, TGF- β , CCL17, EGF, and MMP9. (c) Protein level changes of iNOS. All tests were repeated three times. ( ∗ p < 0.05; † p < 0.01). (d) Flow cytometry analysis of iNOS, CD86, and CD163 proteins.

Article Snippet: LPS (L4391, Sigma) was used with a concentration of 10 ng/mL, SNAP (S-Nitroso-N-Acetyl-D,L-Penicillamine; Sigmal) was used with a concentration of 50 μ M, and cPTIO (2-(4-carboxyphenyl)-4,5-dihydro-4,4,5,5-tetramethyl-1H-imidazolyl-1-oxy-3-oxide, monopotassium salt; Cayman) was used with a concentration of 50 μ M. For staining with antibodies, anti-iNOS-PE (12-5920-80, eBioscience), anti-CD163-PE (bs-2527R-PE, Bioss), and anti-CD86-FITC (bs-1035R-FITC, Bioss) were used.

Techniques: Flow Cytometry

Journal: Molecules

Article Title: The Main Structural Unit Elucidation and Immunomodulatory Activity In Vitro of a Selenium-Enriched Polysaccharide Produced by Pleurotus ostreatus

doi: 10.3390/molecules27082591

Figure Lengend Snippet: Effects of Se-POP-21 on the expression of CD80 ( a ) and CD86 ( b ) in RAW264.7 cells, as measured by flow cytometry. The data are expressed as mean ± SD; ** p < 0.01 compared with the control group.

Article Snippet: CD80 and CD86 antibodies (FITC) were purchased from Sino Biological Inc. (Beijing, China).

Techniques: Expressing, Flow Cytometry